LOCUS       40924_23739       12336 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB718 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12336)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1193..1222
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1229..1258
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1274..1303
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1310..1339
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1358..1482
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1519..1549
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1603..2259
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2604..2906
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2950..3074)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3100..3352
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3386..3402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3410..3426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3434..3464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3479..3500)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3536..3788)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3826..5121)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61211.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3826..5121)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5173..5352)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5858..5882)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6403..7191
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7440..8028
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8214..8354)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8698..8892)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8961..10031)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10463..11089)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaacg gtgaacaaaa
     1201 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     1261 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     1321 aatctccgag gaagacttga acggtagcgc tgttatcaca agtttgtaca aaaaagctga
     1381 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     1441 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
     1501 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1561 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1621 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1681 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1741 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1801 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1861 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1921 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1981 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2041 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2101 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2161 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2221 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     2281 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2341 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2401 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2461 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2521 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2581 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2641 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2701 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2761 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2821 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2881 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2941 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3001 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3061 ttgtacaaag tggtgataac ctagagctcg aatttccccg atcgttcaaa catttggcaa
     3121 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3181 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3241 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3301 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3361 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3481 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     3541 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3601 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     3661 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     3721 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     3781 gaacgatcgg ggaaattcgc ctcgagtgac ttctctgatt gcagactatt tataccaacc
     3841 agcaagaaag tgtcgaagta tgaagcagaa ggcgcaggct agagcctgga aaacgagaag
     3901 atggatgcaa agagactttt ctgatttagg accaaacaac ctcaagtaaa cattgacgag
     3961 tgtcccatct tttgtgccgg tcagtaatcc ggtagcagga tcataccttg ggagacgatg
     4021 gcgcggacat ttcacaatcc cagcaagctg aggaagcgat aatagaaggt tcagaacttg
     4081 aggaagaaag aagatgagga gggtttcact gaagtgaccc agaataccaa caactgccat
     4141 agtcattccg gcgaagactg tgtaagtgtc tccaacaaaa actgaagaag gataccaatt
     4201 gtaagcaagc attgccaagg atgtggccat cagcggttga gtaagaaata tcgagaaggc
     4261 atgagcttgg tgatactcgg gatcaacaga tgctccgatt tgcataacgt tgtgtatcag
     4321 gatagcagcg gcaataacaa ctgtttgacc gatttcaagg ccattcagac cagcgtggat
     4381 gttaatagag tttgtacaaa agacagcaag tagccccata tataacttat atatccttcc
     4441 tagattaagc acttccaagc caatataagc aacaagaggt tttggtataa caatagttgt
     4501 atgaccagca taagccatca aaagtggaag ggtagcaaaa gatggcagga caagttttac
     4561 tctccaaggc acatcaagga catcatctac aaatccaagc aaaatcatga agcaaataga
     4621 tgctagtgct gcattatact ccacaagcca attagaatct tcagtgaagt taaaatactg
     4681 aaatattatc gccacgatca aaaagacaat accaacaaca atacccaatg actctggcac
     4741 tttaatatct ccttgaggcg tgcctctttt gttgatatca aaaccaaaca tgttacgtct
     4801 cagaacgtat ctggcagtaa caggaatcat tttgagagtg acgaaaaacc caaccaagct
     4861 aagccccgcg ttaattagaa tcgatcgctt gagttcatta tcaaccttgt agtgaaaaca
     4921 gagaaggtat agatagagcg aacagagaag agtagagatc acgaagatca ctcctaattt
     4981 cggcggcgcg agacgaaatt cctcaccgga agcagaaacc gtctttcggg tcatcttctg
     5041 ctccgatggc gctgagttgg gttccgacgg gtccggtttg ttgggaattg agatcgatga
     5101 agctctctta cgagctgcca tatcttattg ccccccggga tcagatctgg attgagagtg
     5161 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     5221 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     5281 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
     5341 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     5401 cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct
     5461 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     5521 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     5581 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     5641 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
     5701 gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc
     5761 gtcgagatta atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg
     5821 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     5881 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     5941 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     6001 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     6061 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     6121 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     6181 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     6241 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     6301 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     6361 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     6421 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     6481 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     6541 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     6601 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     6661 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     6721 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     6781 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     6841 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     6901 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     6961 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     7021 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     7081 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     7141 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     7201 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     7261 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     7321 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     7381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7561 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     8221 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     8281 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     8341 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8401 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     8461 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     8521 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     8581 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     8641 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     8701 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     8761 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     8821 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     8881 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     8941 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     9001 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     9061 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     9121 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     9181 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     9241 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     9301 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     9361 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     9421 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     9481 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     9541 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     9601 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     9661 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     9721 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     9781 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     9841 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     9901 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     9961 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
    10021 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
    10081 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
    10141 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
    10201 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
    10261 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
    10321 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
    10381 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
    10441 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
    10501 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
    10561 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
    10621 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
    10681 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
    10741 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
    10801 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
    10861 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
    10921 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
    10981 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
    11041 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
    11101 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
    11161 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
    11221 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
    11281 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
    11341 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
    11401 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
    11461 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
    11521 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
    11581 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
    11641 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
    11701 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
    11761 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
    11821 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
    11881 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
    11941 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
    12001 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
    12061 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
    12121 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
    12181 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
    12241 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
    12301 tggcatgcac atacaaatgg acgaacggat aaacct
//
